Pacific Northwest National Laboratory JBEI (Joint BioEnergy Institute) Post Doc Research Associate in Fungal Molecular Biology in EMERYVILLE California United States in EMERYVILLE, California
Organization and Job ID
Job ID: 309885
Directorate: Energy and Environment
Division: Energy Processes & Materials
Group: Chemical and Biological Processing
We are seeking an independent, energetic fungal molecular biologist to develop fungal platforms for the expression and characterization of lignin degrading enzymes: peroxidases, laccases, etc. The candidate will join our Fungal Biotechnology team that is focused on performing fundamental research and development on fungal heterologous protein expression systems, specifically Aspergillus niger . Such fungi are amenable to industrial-scale production of enzymes relevant to biofuel production. The candidate will have opportunities to collaborate with a large team of leading scientists and engineers in a state-of-the-art facility within the Joint BioEnergy Institute (JBEI). The principal interactions are with the Deconstruction Division that is developing advanced plant biomass deconstruction technologies for production of sugars and lignin components for downstream production of biofuels and bioproducts. The researcher will use expression systems developed in the group to engineer filamentous fungi to produce high titers of known lignin degrading metalloenzymes. This will also involve collaborating with other groups in the Deconstruction Division to characterize these enzymes and develop lignin deconstructing enzyme cocktails. The candidate will have the opportunity to gain exposure to a variety of disciplines through collaborative work on the whole process from biomass to biofuels.
Candidates must have received a PhD within the past five years (60 months) or within the next 8 months from an accredited college or university.
Experience with molecular biology of filamentous fungi or yeasts, including building expression and deletion constructs, transformation, DNA and RNA purification, genomics and transcriptomics.
Ability to design and conduct genetic engineering experiments focused on improving heterologous expression of lignin degrading enzymes in fungi
Some enzymology experience that may include quantitative enzyme assays, statistical analysis of data, protein purification and familiarity with proteomics data analysis.
Perform research in a collaborative manner in the context of processes upstream and downstream of enzymatic biomass deconstruction, including pretreatment of plant feedstocks, enzymatic digestion of pretreated biomass and biological conversion of biomass-derived sugars and aromatics.
Maintain an accurate and detailed laboratory notebook (e-notebooks will be used) of all work performed and submit this information into an electronic database. Ensure that others can duplicate results. Contribute to a safe, respectful and considerate working environment.
Periodically provide written reports and give oral presentations to discuss results and issues.
Ph.D. Biochemistry, Molecular Biology, Genetics, Mycology, Microbiology or related field
3.5 GPA or higher is prefered
Equal Employment Opportunity
Battelle Memorial Institute (BMI) at Pacific Northwest National Laboratory (PNNL) is an Affirmative Action/Equal Opportunity Employer and supports diversity in the workplace. All employment decisions are made without regard to race, color, religion, sex, national origin, age, disability, veteran status, marital or family status, sexual orientation, gender identity, or genetic information. All BMI staff must be able to demonstrate the legal right to work in the United States. BMI is an E-Verify employer. Learn more at jobs.pnnl.gov.
Directorate: Energy & Environment
Job Category: Post-Graduates and Post-Docs
Group: Chem & Biological Processing
Opening Date: 2019-10-01
Closing Date: 2019-11-24